Advances in Enzymology and Related Areas of Molecular Biology (Volume 77)


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Journal of Plant Sciences. Advances in Bioscience and Bioengineering. American Journal of Bioscience and Bioengineering. American Journal of Agriculture and Forestry. American Journal of BioScience. International Journal of Biomedical Materials Research. Ecology and Evolutionary Biology. International Journal of Agricultural Economics. Chemical and Biomolecular Engineering. International Journal of Biomedical Science and Engineering.

Biomedical Statistics and Informatics. Journal of Chemical, Environmental and Biological Engineering. International Journal of Animal Science and Technology. Journal of Diseases and Medicinal Plants. Journal of Drug Design and Medicinal Chemistry. International Journal of Pharmacy and Chemistry. International Journal of Applied Agricultural Sciences.

European Journal of Clinical and Biomedical Sciences. Biomedical Sciences. American Journal of Chemical and Biochemical Engineering. American Journal of Biological and Environmental Statistics. Frontiers in Environmental Microbiology. Advances in Applied Physiology.

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International Journal of Microbiology and Biotechnology. International Journal of Ecotoxicology and Ecobiology. International Journal of Systems Engineering. Bioprocess Engineering. Industrial Engineering. Journal of Biomaterials. World Journal of Food Science and Technology.

American Journal of Entomology. International Journal of Bioorganic Chemistry. International Journal of Photochemistry and Photobiology. Linalool has two enantiomers, R -linalool and S -linalool. Both are known to possess several biological activities in stressed animals. Our previous work revealed that inhalation of R -linalool altered hypothalamic gene expression in rats under stress.

In the present study, we monitored hypothalamic gene expression in restrained rats with and without S -linalool inhalation by DNA microarray.

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The entire gene expression profile showed that inhalation of S -linalool significantly changed the expression levels of hypothalamic genes in the restrained rats. The differentially expressed genes e. These results indicate that S -linalool influences hypothalamic gene expression in restrained rats, and that inhalation of S -linalool under the stressed condition has some effects on stress-related biological responses. PCC To elucidate the scavenging systems of sugar- and lipid-derived reactive carbonyls RCs in the cyanobacterium Synechocystis sp.

PCC S.

It catalyzed the AKR reaction scavenging several lipid-derived RCs, acrolein, propionaldehyde, and crotonaldehyde. These enzymes contribute to scavenging sugar- and lipid-derived RCs as scavenging systems. Plant elongation growth on a day-to-day basis is enhanced under specific photoperiod and temperature conditions. Circadian clock is involved in the temperature adaptive photoperiodic control of plant architecture, including hypocotyl elongation in Arabidopsis thaliana.

In this regulation, phytochrome interacting transcriptional factors, PIF4 and PIF5, are activated at the end of night under short photoperiod or high temperature conditions, due to the coincidence between internal circadian rhythm of the transcripts and external length of dark period time cues.

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Brassinosteroid and gibberellic acid played a positive role in the hypocotyl elongation of seedlings under light and dark cycle conditions. On the other hand, cytokinin and jasmonic acid played an opposite role. In this study, diurnal expresson profile of a gene encoding a sulfotransferase family protein that is involved in the jasmonic acid metabolism, ST2A , was examined. The results of this study support the idea that the circadian clock orchestrates a variety of hormone-signaling pathways to regulate the photoperiod and temperature-dependent morphogenesis in A. Saccharomyces cerevisiae strains engineered previously to produce proteins with mammalian high mannose structures showed severe growth defects and decreased protein productivity.

In strain YAB, derived from one of these strains by a mutagenesis technique based on the disparity theory of evolution, these undesirable phenotypes were alleviated. Here we describe further engineering of YAB with the aim of synthesizing heterologous glycoproteins with Man 5 GlcNAc 2 , an intermediate for the mammalian hybrid and complex type oligosaccharides.

Advances in Enzymology and Related Areas of Molecular Biology, Volume 77

Masaki J. Desferrioxamines E 1 , D2 2 , X1 3 , and X2 4 , four macrocyclic N -hydroxy- N -succinyl diamine-based siderophores, were produced efficiently by heterologous expression of a fusion biosynthetic gene cluster. This expression system consisted of three genes mbsA-C from marine metagenomic DNA and one gene dfoC C from the terrestrial bacterium Erwinia amylovora.

http://leondumoulin.nl/language/comics/fame-katy-perry-graphic-novel.php The first three genes are functional in the production of the common monomers N -hydroxy- N -succinyl cadaverine 5 , HSC and N -hydroxy- N -succinyl putrescine 6 , HSP , whereas dfoC C catalyzes the oligomerization and the macrocyclization reactions of compounds 5 and 6 to form compounds 1 — 4. This fusion gene cluster system provides a convenient expression platform for various biosynthetic genes of HSC-HSP based siderophores by simply switching the fourth gene by the cassette process. Escherichia coli RNase G is involved in the degradation of several mRNAs, including adhE and eno , which encode alcohol dehydrogenase and enolase respectively.

Secondary structure prediction and further site-directed deletion suggested that the stem-loop structure, with a bubble in the stem, is required for RNaseG-dependent degradation of adhE mRNA. We describe a transient dual-luciferase assay combined with a glucocorticoid-inducible system for rice protoplasts. Luciferase genes were efficiently induced by adding 0. This assay system is applicable to studying the translation efficiency of rice by using the luciferase gene harboring tandem copies of an interesting codon at the 5' end.


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These additional chromosomes 2A and 6A would therefore have anonymous genes related to the upregulation of polyphenol production, the antioxidative activities consequently being increased in these MALs. Lily pollen was developed to express HBsAg by Agrobacterium -mediated transformation.

A double prime-boost strategy combining parenteral and oral HBsAg boosters was found to increase antibody titer levels fold, as compared to a single process of priming and boosting. Lily pollen may prove a tool for oral vaccine development. Expression and Characterization of a Thermostable Acetylxylan Esterase from Caldanaerobacter subterraneus subsp. A thermostable acetylxylan esterase gene, TTE, which catalyzes the deacetylation of cellulose acetate, was cloned from the genome of Caldanaerobacter subterraneus subsp.

The pH and temperature optima were 8. The esterase was inhibited by phenylmethylsulfonyl fluoride.

A mixture of the esterase and cellulolytic enzymes efficiently degraded insoluble cellulose acetate with a higher degree of substitution. Peach trees bear either white- or yellow-flesh fruit.

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We found that Japanese peach cultivars have two types of mutation in a carotenoid catabolic gene, CCD4 : the insertion of a retrotransposon, and a frame shift in the microsatellite sequences of the first exon. CCD4 in yellow-flesh peaches was disrupted by these mutations. However, contrary to fosfomycin, these compounds showed potent inhibitory activities against MurA overexpressing Escherichia coli , especially in the presence of UDP-GlcNAc.

These observations suggest that these compounds induced bacterial cell death not through a MurA malfunction, but in such a MurA-mediated indirect manner as the inhibition of other Mur enzymes. The levels of food allergens in gamma-irradiated soybean 0, 2. After 3 months of storage, Coomassie brilliant blue CBB staining indicated similar total protein profiles among the treatments, but that some proteins were degraded by irradiation at high doses. Competitive inhibition ELISA analyses suggested that there were no significant changes in the allergen contents, except for a decrease in the soybean trypsin inhibitor.

The patient IgE binding allergenic protein patterns were not changed by irradiation up to 30 kGy. ELISA using patient serum also revealed that the IgE reactivity to the irradiated soybean extract did not increase from the level of the control, but that the reactivity to some patient serum IgE was significantly decreased by irradiation. However, they are promising in terms of their bio-functionality such as preventing diabetes. Miso soybean paste is a yeast-fermented food, made from steamed soybeans, salt, and inoculated cereals known as koji , made from rice, barley, or soybeans.

We investigated the effects of soaking ae mutant rice cultivars in a miso suspension. Rice grains cooked after soaking in the miso suspension were less hard and more sticky than those cooked after soaking in water. Strain ABRG The cyanobacterium semi-filamentous multicellular strain ABRG undergoes cell lysis as a unique feature that occurs due to growth condition changes from normal cultivation with shaking to static cultivation without shaking in liquid culture Nishizawa et al. Microscopic observation and energy dispersive X-ray spectrometer EDX analysis have revealed that lysis is involved in the accumulation of polyphosphate compounds and the disintegration of thylakoid membranes in cells.

Moreover, stress induced by salts, osmotic pressure with sucrose, and the depletion of nitrogen or phosphate in cultures also induced ABRG cell lysis. Based on these results, we discuss lysis and its utilization in the biotechnology industry. The crude biosurfactant had the ability to reduce the surface tension from Physicochemical analysis of the crude biosurfactant revealed that it had wide ranges of optimum pH and pH stability at 6—9 and 3—10 respectively.

This revealed that the biosurfactant mainly contained sophorolipids in C MW and Cdiaceltylated MW forms. N -Acylhomoserine lactones AHLs function as quorum-sensing signaling molecules in many Gram-negative bacteria. We isolated a total of bacterial strains from activated sludge obtained from seven sewage treatment plants in Tochigi Prefecture, Japan, and screened for AHL-producing and degrading strains.

Advances in Enzymology and Related Areas of Molecular Biology (Volume 77) Advances in Enzymology and Related Areas of Molecular Biology (Volume 77)
Advances in Enzymology and Related Areas of Molecular Biology (Volume 77) Advances in Enzymology and Related Areas of Molecular Biology (Volume 77)
Advances in Enzymology and Related Areas of Molecular Biology (Volume 77) Advances in Enzymology and Related Areas of Molecular Biology (Volume 77)
Advances in Enzymology and Related Areas of Molecular Biology (Volume 77) Advances in Enzymology and Related Areas of Molecular Biology (Volume 77)
Advances in Enzymology and Related Areas of Molecular Biology (Volume 77) Advances in Enzymology and Related Areas of Molecular Biology (Volume 77)
Advances in Enzymology and Related Areas of Molecular Biology (Volume 77) Advances in Enzymology and Related Areas of Molecular Biology (Volume 77)
Advances in Enzymology and Related Areas of Molecular Biology (Volume 77) Advances in Enzymology and Related Areas of Molecular Biology (Volume 77)
Advances in Enzymology and Related Areas of Molecular Biology (Volume 77) Advances in Enzymology and Related Areas of Molecular Biology (Volume 77)
Advances in Enzymology and Related Areas of Molecular Biology (Volume 77)

Related Advances in Enzymology and Related Areas of Molecular Biology (Volume 77)



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